三株递呈猪流行性腹泻病毒S基因的减毒鼠伤寒沙门菌的生物学特性研究

doi:10.11843/j.issn.0366-6964.2015.02.015

Abstract

Abstract:

To explore the feasibility of attenuated Salmonella typhimurium as the carrier of porcine epidemic diarrhea virus (PEDV) S gene，the biological characteristics of three recombinant attenuated Salmonella strains harboring varying length of PEDV S gene were researched.The biological characteristics research of SL7207(pVAX-S_499-650) (456 bp，encoding 499-650 amino acid of N-termanal S protein，SL7207(pVAX- S_499-789) (873 bp，encoding 499-789 amino acid of N-termanal S protein)，SL7207(pVAXD-S_1-789) (2 367 bp，encoding 1-789 amino acid of N-termanal S protein) which harboring PEDV S genes in varying length plasmid DNA showed：SL7207(pVAX-S_499-650)，SL7207(pVAX-S_499-789) and SL7207(pVAXD-S_1-789) were steady in vivo/vitro；the target genes could be transcribed in the ileum tissue of mouse；recombinant strains could colonize in liver and spleen of mice and gradually eliminated by host；safety to BALB/c mouse at the dosage of 1×10⁹ CFU by oral administration.All of this，demonstrating the attenuated S.typhimurium could be the carrier of PEDV S gene and laying the foundation for evaluating the immunogenicity of recombinant attenuated Salmonella strains in piglet.

CLC Number:

S858.285.3

LIANG En-tao，CHEN Jie,HUANG Xiao-bo，WEN Xin-tian，CAO San-jie，WU Xue-jing，ZHU Shu-quan. Study on the Biological Characteristics of Three Attenuated Salmonella typhimurium Strains Harboring Porcine Epidemic Diarrhea Virus S Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(2): 279-287.

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Study on the Biological Characteristics of Three Attenuated Salmonella typhimurium Strains Harboring Porcine Epidemic Diarrhea Virus S Gene

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